Part:BBa_K5487005

The composite part, consisting of the T7 Promoter, lac operator, RBS, Lpp-OmpA, and sfGFP, is designed for the controlled expression and membrane targeting of superfolder green fluorescent protein (sfGFP) in bacterial systems. The T7 Promoter, under the regulation of the lac operator, ensures inducible and robust transcription. The RBS initiates efficient translation of the fused Lpp-OmpA and sfGFP, with Lpp-OmpA directing sfGFP to the bacterial membrane. Successful expression and membrane localization are confirmed through GFP fluorescence, verifying the functionality of the Lpp-OmpA signal peptide. This construct serves as a valuable tool for studying membrane protein expression and for educational demonstrations of protein localization in synthetic biology applications.